Ashok Mulchandani
Professor of Chemical Engineering
|
Molecular Biology
Ph.D. McGill University, 1985
VOICE: 951-827-6419 |
Development of biological transducer- (enzyme, whole cell, antibody, receptor, tissue, etc.) based sensitive, selective, rapid and economical analytical tools for applications in health care, environmental monitoring, food industry and bioprocess monitoring and control are the major emphases of our research program. Presently on going projects are: (1) development and application of enzyme and microbial biosensors for selective, rapid and direct monitoring of organophosphate pesticides and nerve agents; (2) development of a highly selective and sensitive receptor protein based biosensor for monitoring of anticancer drug paclitaxel (taxol); (3) detection of environmental pathogens using novel DNA amplification techniques; (4) assessment of bio-available benzene, toluene, ethyl benzene and xylene from underground leaking gasoline tanks using catabolic reporter bacterium; and (5) development of integrated amperometric enzyme electrodes based on electrochemical polymers for monitoring and control of mammalian and hybridoma cell culture processes for production of therapeutic proteins and monoclonal antibodies, respectively.
Another active research area of our group involves the development of novel bioprocesses and immobilized cell bioreactors for biodetoxification of highly toxic organic chemicals. Currently active projects focus on the application of (1) the novel recombinant Escherichia coli expressing the enzyme responsible for the biodetoxification of organophosphate pesticides and nerve agents on its cell surface, thereby eliminating mass transport problems normally encountered in systems using enzyme expressed intracellularly, in immobilized cell bioreactor and (2) removal of heavy metals using recombinant cells expressing synthetic phytochelatins on the cell surface.
SELECTED PUBLICATIONS
Wang, J., Chen, L., Mulchandani, A., Mulchandani, P. and Chen, W. 1999. Remote biosensor for in-situ monitoring of organophosphate nerve agents. Electroanalysis (Accepted).
Roger, K.R., Wang, Y., Mulchandani, A., Mulchandani, P., Chen, W. 1999. Organophosphorus hydrolase-based fluorescence assay for organophosphate pesticides. Biotech. Prog.(Accepted).
Mulchandani, A., Mulchandani, P., Chen, W., Wang, J., Chen, L. 1999. Amperometric thick-film strip electrodes for monitoring organophosphate nerve agents based on immobilized organophosphorus hydrolase. Anal. Chem. (Accepted).
Mulchandani, A., Pan, S. and Chen, W. 1999. Fiber-optic biosensor for direct determination of organophosphate nerve agents. Biotechnol. Progress 15, 130-134..
Chen, W. Bruhlmann, F., Richins, R.D., Mulchandani, A. 1999. Engineering of improved microbes and enzymes for bioremediation. Curr. Opinion Biotechnol. 10, 137-141.
Mulchandani, A., Kaneva, I. and Chen, W. 1999. Detoxification of organophosphate pesticides by immobilized Escherichia coli expressing organophosphorus hydrolase on cell surface. Biotechnol. Bioeng. 63, 216-223..
Mulchandani, A. and Pan, S. 1999. Ferrocene conjugated m-phenylenediamine conducting polymer-incorporated peroxidase biosensors. Anal. Biochem. 267, 141-147.
Mulchandani, A., Kaneva, I. and Chen, W. 1998. Detoxification of organophosphate pesticides by immobilized Escherichia coli expressing organophosphorus hydrolase on cell surface. In Proceedings of the 1998 AIChE Conference on Pollution Prevention and Environmental Risk Reduction.
Mulchandani, A., Kaneva, I. and Chen, W. Microbial biosensor for direct determination of organophosphate nerve agents using recombinant Escherichia coli with surface-expressed organophosphorus hydrolase. 2. Fiber-optic microbial biosensor. Anal. Chem. 70, 5042-5046.
Mulchandani, A., Mulchandani, P. Kaneva, I. and W. Chen. 1998. Biosensor for direct determination of organophosphate nerve agents using recombinant Escherichia coli with surface-expressed organophosphorus hydrolase. 1. Potentiometric microbial electrode. Anal. Chem. 70, 4140-4145.
Mulchandani, P., Mulchandani, A., Kaneva, I. and Chen, W. 1999. Biosensor for direct determination of organophosphate nerve agents. 1. Potentiometric enzyme electrode. Biosens. Bioelectron., 14, 77-85.
Mulchandani, A., Mulchandani, P., Chauhan, S., Kaneva, I. and Chen, W. 1998. A potentiometric microbial biosensor for direct determination of organophosphate nerve agents. Electroanalysis 10, 733-737.
Mulchandani, A., Mulchandani, P, Chen, W. Enzyme biosensor for determination of organophosphates. Field Anal. Chem. Technol. 2, 363-369.
Kaneva, I., Mulchandani, P., Richins, R., Mulchandani, A., and Chen, W. 1998. Biodetoxification and monitoring of organophosphorus nerve agents by surface expressed organophosphorus hydrolase. In Proceedings of the 1997 ERDEC Scientific Conference on Chemical & Biological Defense Research, 18-21 November 1997, ERDEC-SP-063, 251-255.
Kaneva, I., Mulchandani, A. and Chen, W. 1998. Optimization of parathion degradation using surface expressed organophosphorus hydrolase. Biotechnol. Prog. 14, 275-278.
Chen, W. and Mulchandani, A. 1998. The use of "live biocatalysts" for pesticide detoxification. TIBTECH.16,71-76.
Mulchandani, A. Principles of enzyme biosensors. 1998. In Enzyme and Microbial Biosensors: Protocols and Techniques, Mulchandani, A and Rogers, K.R. (Eds.), Humana Press, NJ. pp. 3-14.
Deshusses, M.A., Chen, W., Mulchandani, A. and Dunn, I.J. 1997. Innovative bioreactors. Curr. Opinion Biotechnol. 8, 165-168.
Suye, S., Tandel, S. and Mulchandani, A. 1997. A novel receptor protein-based assay for quantitative determination of taxol. Anal. Chem., 69, 3633-3635.
Richins, R., Kaneva, I., Mulchandani, A. and Chen, W. 1997. Biodegradation of organophosphorus pesticides by surface-expressed organophosphorus hydrolase. Nat. Biotechnol. 15, 984-987.
35. Mulchandani, A. and Wang, C-L. 1996 Bienzyme sensors based on poly(anilinomethylferrocene)-modified electrode. Electroanalysis, 8, 414-419.
34. Mulchandani, A. and Bassi, A. S. 1996. Determination of glutamine and glutamic acid in mammalian cell cultures using tetrathiafulvalene modified enzyme electrodes. Biosens. Bioelectron., 11, 271-280.
33. Zhou, W. and Mulchandani, A. 1995. Recent advances in bioprocess monitoring and control. In Biosensors and Chemical Sensor Technology: Process Monitoring and Control, Rogers, K., Mulchandani, A. and Zhou, W. (eds.), ACS Symposium Series No. 613, ACS, Washington, DC, pp. 88-98.
32. Rogers, K.R. and Mulchandani, A. 1995. Biomolecular sensing for bioprocess and environmental monitoring applications. In Biosensors and Chemical Sensor Technology: Process Monitoring and Control, Rogers, K., Mulchandani, A. and Zhou, W. (eds.), ACS Symposium Series No. 613, ACS, Washington, DC, pp. 2-8.
31. Mulchandani, A. and Barrows, L. 1995. Chemically modified electrode for hydrogen peroxide measurement by reduction at low potential. In Biosensors and Chemical Sensor Technology: Process Monitoring and Control, Rogers, K., Mulchandani, A. and Zhou, W. (eds.), ACS Symposium Series No. 613, ACS, Washington, DC, pp. 61-69.
30. Mulchandani, A. and Bassi, A. S. 1995. Principles and applications of biosensors for bioprocess monitoring and control. Crit. Rev. Biotechnol. 15, 105-124.
29. Wang, C-L. and Mulchandani, A. 1995. Ferrocene conjugated polyaniline modified enzyme electrodes for determination of peroxides in organic media. Anal. Chem. 67, 1109-1114.
28. Mulchandani, A. and Rudolph, D. C. 1995. Amperometric determination of lipid hydroperoxides. Anal. Biochem., 227, 277-282.
27. Mulchandani, A., Bassi, A. S.and Nguyen, A. 1995. Development of a tetrathiafulvalene mediated biosensor for the specific determination L-lactate in dairy products. J. Food Sci., 60, 74-78.
26. Mulchandani, A., Wang, C-L. and Weetall, H. H. 1995. Amperometric detection of peroxides with poly(anilinomethylferrocene) modified enzyme electrodes. Anal. Chem., 67, 94-100.
BOOKS
Mulchandani, A. and Rogers, K. R. (eds.) 1998. Enzyme and Microbial Biosensors: Techniques and Protocols. Humana Press, Totowa, NJ.
Mulchandani, A. and Rogers, K. R. (eds.) 1998. Affinity Biosensor Techniques and Protocols. Humana Press, Totowa, NJ.
Rogers, K. R., Mulchandani, A. and Zhou, W (eds.). 1995. Biosensors and Chemical Sensors Technology. ACS Symposium Series No. 613, American Chemical Society, Washington, DC.
Mulchandani, A. and Sadik, O.A. (eds.) Recent Advances in Environmental Chemical Sensors and Biosensors. (In Preparation).
PATENTS
Mulchandani, A. and Wang, C-L. Chemically modified electrodes for amperometric determination of hydrogen and organic peroxides in aqueous and nonaqueous media at low applied potential. (Application Filed).